Etiology and Host Range of a Closterovirus Associated with Plum Bark Necrosis-Stem Pitting Disease
نویسنده
چکیده
Plum bark necrosis-stem pitting (PBNSP) disease was first observed in 1986 affecting plum (Prunus salicina Lindl.) cv. Black Beaut trees in two orchards in Dinuba, CA (8). All of the Black Beaut plum trees had been whip-grafted with a single cultivar Wickson plum scion per tree to serve as a pollenizer, and all Wickson grafts failed. A year later, the same trees were grafted a second time with another source of Wickson scion wood, which successfully callused and grew. Meanwhile, scaffold branches with the failed grafts began to exude copious amounts of dark-colored gum and developed necrotic bark and stem pitting symptoms. Disease incidence approached 100%, and both sites were eventually replanted. We hypothesized the first collection of Wickson scions was diseased and the second healthy. Subsequent graft-transmission tests performed at University of California at Davis (UCD) proved the infectious nature of PBNSP (8). In the present study, an associated virus, and the putative causal agent, was identified as a Closterovirus and designated as Plum bark necrosis stem-pitting associated virus (PBNSPaV). The host range, symptoms induced, and development of a reverse transcriptase-polymerase chain reaction (RT-PCR) assay for the associated Closterovirus are described.
منابع مشابه
Complete Genomic Characterization of Plum bark necrosis stem pitting–associated virus Infecting Sweet Cherry in China
Plum bark necrosis stem pitting-associated virus (PBNSPaV) causes the plum bark necrosis stem pitting-associated disease. We obtained the complete genome of a PBNSPaV isolate (PBNSPaV-TA) using small RNA deep sequencing followed by overlapping RT-PCR. To our knowledge, this is the first report of a completed genome of PBNSPaV identified from cherry trees.
متن کاملSome aspects of biology and host range of Biscogniauxia mediterranea, one of the causal agent of oak charcoal disease
Field surveys in Zagros forests located in western Iran were done to study some aspects of biology and host range of Biscogniauxia mediterranea during 2014-2016. The ability of its conidia and ascospores to cause the disease was evaluated under greenhouse condition. Ascospore viability and development of anamorph and teleomorph were surveyed under different conditions. The pathogen host range w...
متن کاملEnhancement or attenuation of disease by deletion of genes from Citrus tristeza virus.
Stem pitting is a common virus-induced disease of perennial woody plants induced by a range of different viruses. The phenotype results from sporadic areas of the stem in which normal xylem and phloem development is prevented during growth of stems. These alterations interfere with carbohydrate transport, resulting in reduced plant growth and yield. Citrus tristeza virus (CTV), a phloem-limited...
متن کاملGenetic Diversity and Molecular Evolution of Plum bark necrosis stem pitting-associated virus from China
Plum bark necrosis stem pitting-associated virus (PBNSPaV), a member of the genus Ampelovirus in the family Closteroviridae, infects different Prunus species and has a worldwide distribution. Yet the population structure and genetic diversity of the virus is still unclear. In this study, sequence analyses of a partial heat shock protein 70 homolog (HSP70h) gene and coat protein (CP) gene of PBN...
متن کاملSerological Detection of Grapevine Associated Closteroviruses in Infected Grapevine Cultivars
Grapevine leafroll (LR) and corky bark (CB) diseases occur wherever grapevines are grown and are associated with undesirable viticultural effects which include delayed ripening of fruit, reduced yield, altered fruit pigmentation, and reduced accumulation of sugar (6,24). Rootstockscion incompatibility (RSI) is a disease syndrome characterized by incompatibility at the graft union resulting in s...
متن کامل