Etiology and Host Range of a Closterovirus Associated with Plum Bark Necrosis-Stem Pitting Disease

نویسنده

  • Diana B. Marini
چکیده

Plum bark necrosis-stem pitting (PBNSP) disease was first observed in 1986 affecting plum (Prunus salicina Lindl.) cv. Black Beaut trees in two orchards in Dinuba, CA (8). All of the Black Beaut plum trees had been whip-grafted with a single cultivar Wickson plum scion per tree to serve as a pollenizer, and all Wickson grafts failed. A year later, the same trees were grafted a second time with another source of Wickson scion wood, which successfully callused and grew. Meanwhile, scaffold branches with the failed grafts began to exude copious amounts of dark-colored gum and developed necrotic bark and stem pitting symptoms. Disease incidence approached 100%, and both sites were eventually replanted. We hypothesized the first collection of Wickson scions was diseased and the second healthy. Subsequent graft-transmission tests performed at University of California at Davis (UCD) proved the infectious nature of PBNSP (8). In the present study, an associated virus, and the putative causal agent, was identified as a Closterovirus and designated as Plum bark necrosis stem-pitting associated virus (PBNSPaV). The host range, symptoms induced, and development of a reverse transcriptase-polymerase chain reaction (RT-PCR) assay for the associated Closterovirus are described.

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تاریخ انتشار 2002